Matthews, Kent. the economics of banking kent matthews and john thompson, the economics of banking matthews and .An in vitro model for a human peripheral nerve/bone interface: differentiation of Schwann cells in a microfluidic channel.
The functional integration of peripheral nerves and bone is a prerequisite for the successful treatment of peripheral nerve injury or for the design of nerve guidance channels for intra-bony nerve regeneration. In this study we describe a model for a peripheral nerve/bone interface using bone marrow stromal cells (BMSCs) to induce the Schwann cell phenotype in an in vitro model. The human mesenchymal BMSCs were encapsulated in a collagen matrix and grown for 7 days in a microfluidic channel. They were then grown for a further 4 weeks in an in vitro Schwann cell-inducing medium (neural induction medium). During culture, the encapsulated cells were connected to the two reservoirs of the microfluidic channel by the microgrooves on its walls. Following this, the cells were maintained in a nerve-guidance medium. Morphological and immunocytochemical analyses demonstrated that the BMSCs could undergo a transition from a fibroblast-like to a Schwann cell-like phenotype, forming a process that was terminated by a mesenchymal cell. These phenotypic changes were associated with changes in the Schwann cell-specific marker S100β and a change in cell morphology. In addition, the BMSCs were shown to express the neural markers Nestin, Vimentin, NFH, GFAP and peripherin. Finally, an in vitro analysis of the neurite outgrowth potential showed that the cells had the ability to grow neurites and a significant percentage of these neurites were present at the ends of the BMSCs, which also expressed NFH. The model described in this study could be used to study the mechanisms involved in nerve-bone integration as well as in the design of nerve guidance channels.Secrets of Success in the Nonprofit Sector – From CEO to Board Member
Guest post by Hallie Wentz
This post is part of our ongoing series, Secrets of Success in the Nonprofit Sector, highlighting interviews with other nonprofit executives and board members. You’ll find more blogs in this series on the series’ Facebook page and on the web: The NPA Blog.
In the post below, Hallie Wentz, CEO and co-founder be359ba680
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